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A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
Published on: June 6, 2018
[Cloning of AE1-c-end cDNA and construction of its expression plasmid for yeast two-hybrid system]
Hongtao Li1, Guohui Fu, Yong Qin
1Department of Pathophysiology, Harbin Medical University, Harbin 150086.
Abstract:
In this study, about 350 bp cDNA fragment was amplified by PCR. After being sequenced, the AE1-c-end gene fragment was cloned into EcoR I-Pst I site of pGADT7 to form AD ends in the yeast two-hybrid system. The recombinant plasmid was transformed into yeast AH109, and the expression in the yeast was observed. The results demonstrate that AE1-c-end was obtained. pGADT7-AE1-c-end has no toxic effect on the yeast. It can serve as a target gene of yeast two-hybrid system.
