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Related Experiment Videos

A rapid and precise method for determining theophylline in serum.

C S Frings, R C Keefer, J M Saloom

    Clinical Toxicology
    |January 1, 1975
    PubMed
    Summary

    A new spectrophotometric method accurately measures serum theophylline levels. This rapid and sensitive technique avoids interference from barbiturates, making it ideal for routine clinical monitoring.

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    Area of Science:

    • Clinical Chemistry
    • Analytical Chemistry
    • Pharmacology

    Background:

    • Accurate monitoring of serum theophylline concentrations is crucial for patient management.
    • Existing ultraviolet spectrometric methods for theophylline analysis are often affected by interfering substances like barbiturates.

    Purpose of the Study:

    • To develop and validate a robust spectrophotometric method for routine serum theophylline determination.
    • To address the limitations of current methods, particularly interference from barbiturates.

    Main Methods:

    • A novel spectrophotometric assay was developed for quantifying theophylline in serum.
    • Method validation included assessing recovery, linearity, and interference from common substances.
    • Comparison with existing methods was performed to evaluate performance characteristics.

    Main Results:

    • The developed method demonstrated near 100% recovery of theophylline from serum samples.
    • Significant interference from barbiturates, a common issue with other methods, was not observed.
    • The procedure is rapid, completing in under 45 minutes, and offers high sensitivity.

    Conclusions:

    • This spectrophotometric method provides a reliable, sensitive, and rapid approach for routine serum theophylline monitoring.
    • The method's robustness against barbiturate interference enhances its clinical utility.
    • It represents an improvement over existing spectrophotometric techniques for theophylline analysis.

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