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Histological study of conjunctiva-associated lymphoid tissue in mouse
Tohru Sakimoto1, Jun Shoji, Noriko Inada
1Department of Ophthalmology, Nihon University School of Medicine, Tokyo, Japan.
Purpose:
To investigate conjunctiva-associated lymphoid tissue (CALT) in the mouse conjunctiva by histological methods.
Methods:
The presumed follicular tissue in the conjunctiva of normal mice, age ranging from 4 to 6 weeks, was histologically investigated by the hematoxylin-eosin staining method. Next, we treated the mice with topical instillation of a combined solution of ovalbumin and cholera toxin B to investigate the morphological changes of conjunctival follicles to antigen challenge. The treated mice underwent sequential clinical examinations, and the conjunctival follicular tissue was examined by an immunohistochemical method using anti-CD4 antibody, anti-CD8 antibody, and anti-S-100 antibody.
Results:
Follicular tissue was present on the mouse nictitating membrane. Both size and number of follicular tissue areas increased with topical ovalbumin treatment. Immunohistochemical study revealed CD4, CD8, and S-100 positive cells in the follicular tissue. The epithelial layer, corresponding to follicular tissue, demonstrated intra-epithelial pocket and the presence of CD4-positive cells in the intra-epithelial pocket.
Conclusion:
Follicular tissue at the nictitating membrane is CALT in the mouse.