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Reliability and significance of measurements of a-wave latency in rats
Eriko Fujiwara1, Hui Qiu, Mu Liu
1Department of Ophthalmology, Fukuoka University School of Medicine, Fukuoka, Japan.
Purpose:
To determine whether measurements of the a-wave latency of the electroretinogram (ERG) can be made as reliably as that of the implicit time (IT) in rats. In addition, to determine the relationship between the potential level selected for the latency and the baseline potential level.
Methods:
ERGs, elicited by different stimulus intensities, were recorded from Long-Evans rats. The a-wave latency was determined by measuring the time between the stimulus onset and the beginning of the negative-going a-wave, and the IT was measured as the time between the stimulus onset and the peak of the a-wave. To test the reliability of the measurements of the latency, the a-wave latency and the IT were measured by three independent observers for the same 15 ERGs.
Results:
The mean a-wave latency was approximately 14 milliseconds, and the mean a-wave implicit time was approximately 36 milliseconds. The mean of the a-wave latency and the IT, as measured by the three observers, were within 1 millisecond of each other. The coefficient of variation was as good for the latency as for the IT of the a-wave. The potential level selected for the latency was lower than the mean baseline potential level by 1 to 2 standard deviations.
Conclusions:
Selection of the a-wave latencies can be made as reliably as that for the IT. Because the a-wave latency is not affected by the activity of the second order neurons, the latency is a better measure than the IT of the time course of the a-wave.
