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Related Experiment Videos

Cell transformation by v-Jun deactivates ERK MAP kinase signalling.

Elizabeth J Black1, Mark Walker, William Clark

  • 1Beatson Institute for Cancer Research, Cancer Research UK Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK.

Oncogene
|September 13, 2002
PubMed
Summary

v-Jun transformation disrupts cell cycle control by inhibiting the ERK pathway. This involves reduced ERK activity and impaired signaling, impacting cell growth and potentially contributing to oncogenesis.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Oncogenesis

Background:

  • v-Jun accelerates G1 progression and S phase entry without growth factors.
  • Growth factor-dependent ERK MAP kinase signaling regulates the G1/S transition.

Purpose of the Study:

  • Investigate if aberrant ERK regulation contributes to v-Jun-induced cell cycle deregulation.
  • Determine the effects of v-Jun transformation on ERK signaling pathways.

Main Methods:

  • Biochemical analysis of ERK pathway components.
  • Assessing ERK activity and responsiveness to various agonists in v-Jun transformed cells.

Main Results:

  • v-Jun transformed cells show reduced basal and stimulated ERK activity.

Related Experiment Videos

  • ERK signaling becomes refractory to serum, LPA, and EGF but partially responsive to TPA.
  • Defects linked to inefficient Ras-Raf signal propagation and increased MAP kinase phosphatase activity.

    • Conclusions:

    • v-Jun transformation antagonizes ERK signaling at multiple levels.
    • Altered cell physiology in v-Jun transformed cells impacts ERK signaling.
    • This phenotype may have significance in v-Jun-mediated oncogenesis.