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The use of MassARRAY technology for high throughput genotyping.

Christian Jurinke¹, Dirk van den Boom, Charles R Cantor

¹SEQUENOM, Inc, San Diego, CA 92121, USA. cjurinke@sequenom.com

Advances in Biochemical Engineering/Biotechnology

|September 14, 2002

Summary

Mass spectrometry (MS) has evolved into a routine tool for genotyping, particularly for analyzing single-nucleotide-polymorphisms (SNPs). Time-of-flight MS and MassARRAY technology enable high-throughput SNP analysis in biological sciences.

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Area of Science:

Bioanalytical Sciences
Genomic Sciences

Background:

Mass spectrometry (MS) has transitioned from an expert-only technique to a routine method in biological sciences.
Single-nucleotide-polymorphisms (SNPs) are crucial for understanding intraspecies sequence diversity and genomic variation.

Purpose of the Study:

To explore the role of MS in genotyping.
To illustrate the evolution of MS into a routine bioanalytical method.
To highlight the application of time-of-flight (TOF) MS for SNP analysis.

Main Methods:

Review of the evolution of MS in bioanalytical sciences and early MALDI TOF MS protocols.
Overview of intraspecies sequence diversity and the importance of SNPs.
Exploration of the advantages of MS in modern bioanalytical sciences.

Related Experiment Videos

Description of MassARRAY technology for high-throughput SNP analysis.

Main Results:

MS, particularly TOF-based devices, is effective for SNP genotyping.
The MassARRAY system offers automated, high-throughput SNP analysis.
MS has become a key technology in modern bioanalytical and genomic sciences.

Conclusions:

Mass spectrometry is a versatile detection method for genotyping applications.
The evolution of MS has made it accessible for routine SNP analysis.
Advanced systems like MassARRAY facilitate high-throughput genomic studies.