Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A Short-Term Decrease in Nitrogenase Activity (C2H2 Reduction) Is Induced by Exposure of Soybean Shoots to Their CO2

R. Vidal1, A. Gerbaud, D. Vidal

  • 1Laboratoire de Recherche sur les Symbiotes des Racines, Institut National de la Recherche Agronomique, Place Viala, 34060 Montpellier, France (R.V., A.G., J.-J.D.).

Plant Physiology
|August 1, 1995
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same journal

Anther Middle Layer Fatty Acid Synthesis is Essential for Pollen Development and Fertility Restoration at Low Temperature in Arabidopsis.

Plant physiology·2026
Same journal

GmSNAT1 enhances cold tolerance in soybean by promoting melatonin biosynthesis and interacting with GmPSYR1.

Plant physiology·2026
Same journal

Hydraulic architecture traits influence the co-optimization of efficiency and safety in cycads.

Plant physiology·2026
Same journal

SlWRKY6 Mediates H2S Signaling and SlGRF1-SlGIF2 Module to Coordinately Regulate Plant Growth, Fruit yield and Ripening in Tomato.

Plant physiology·2026
Same journal

Blocking miR439 enhances rice disease resistance and yield.

Plant physiology·2026
Same journal

Temperature responses of root carbon use efficiency are linked to cortical cell expansion.

Plant physiology·2026

Soybean photosynthesis and nitrogenase activity (ARA) decline at low CO2 levels. Increased oxygen partially restored ARA, suggesting reduced nodule oxygen permeability limits nitrogen fixation.

Area of Science:

  • Plant Physiology
  • Biochemistry
  • Agricultural Science

Background:

  • Photosynthesis is crucial for plant growth and energy production.
  • Nitrogenase acetylene-reducing activity (ARA) is a key indicator of nitrogen fixation in legumes.
  • Environmental factors like CO2 concentration can significantly impact plant physiological processes.

Purpose of the Study:

  • To investigate the effects of CO2 compensation point exposure on soybean photosynthesis and nitrogenase activity.
  • To determine the role of oxygen availability in modulating nitrogenase activity under low CO2 conditions.

Main Methods:

  • Soybean plants (Glycine max) were exposed to 60 µL L⁻¹ CO2 (CO2 compensation point) for 48 hours.
  • Photosynthesis, transpiration, and nitrogenase acetylene-reducing activity (ARA) were measured.

Related Experiment Videos

  • ARA was assessed under varying oxygen partial pressures (20 kPa and 40 kPa).

    • Main Results:

    • Low CO2 significantly inhibited ARA, reducing it to 20% of control rates within 38 hours.
    • Net photosynthesis ceased, and transpiration decreased by 20% under low CO2.
    • Increasing oxygen partial pressure to 40 kPa restored ARA to levels comparable to control plants.

    Conclusions:

    • Exposure to the CO2 compensation point severely impairs nitrogenase activity in soybeans.
    • Reduced nodule permeability to oxygen is the likely cause of ARA inhibition under low CO2.
    • Oxygen availability is a critical factor in regulating nitrogen fixation in legumes under stress conditions.