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Related Experiment Videos

The Function of Ascorbic Acid in Photosynthetic Phosphorylation.

G. Forti1, G. Elli

  • 1Centro di Studio Consiglio Nazionale delle Ricerche sulla Biologia Cellulare e Molecolare delle Piante, Dipartimento di Biologia, Universita di Milano, Via Celoria 26, Milano 20133, Italy.

Plant Physiology
|December 1, 1995
PubMed
Summary

Ascorbate enhances ATP synthesis in spinach thylakoids by accepting electrons. This light-dependent process involves electron transport from water to monodehydroascorbate, boosting ATP production.

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Stimulation of Photophosphorylation by Ascorbate as a Function of Light Intensity.

Plant physiologyยท1996
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Area of Science:

  • Plant Physiology
  • Photosynthesis Research
  • Biochemistry

Background:

  • Spinach thylakoids generate reactive oxygen species (ROS) like O2.- and H2O2 during the Mehler reaction.
  • Ascorbate peroxidase utilizes H2O2, linking ROS metabolism to ascorbate oxidation.
  • Understanding electron transport pathways is crucial for photosynthesis research.

Purpose of the Study:

  • To investigate the role of ascorbate in light-dependent electron transport and ATP synthesis in isolated spinach thylakoids.
  • To quantify the effect of ascorbate on the Mehler reaction and associated ATP production.
  • To elucidate the electron transport scheme involving ascorbate and monodehydroascorbate.

Main Methods:

  • Isolation of spinach (Spinacia oleracea) thylakoids.

Related Experiment Videos

  • Measurement of light-dependent oxygen exchange and ATP synthesis.
  • Analysis of electron transport coupled to ascorbate and monodehydroascorbate reduction.

    • Main Results:

    • Ascorbate is oxidized to monodehydroascorbate by O2.- and H2O2 in the Mehler reaction.
    • Light-dependent electron transport from water to monodehydroascorbate is coupled to ATP formation with an ATP:O2 ratio of 2.
    • Ascorbate addition results in near-zero net O2 exchange and increases ATP synthesis 2-3 fold.

    Conclusions:

    • Ascorbate acts as an efficient electron acceptor in the Mehler reaction, enhancing ATP synthesis.
    • The Mehler reaction, when coupled with ascorbate reduction, represents a significant pathway for phot ATP production.
    • A detailed scheme of electron transport involving ascorbate and monodehydroascorbate in thylakoids is proposed.