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Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
Published on: February 17, 2017
A possible mechanism for the exchange of transferrin-67Ga complex to heparan sulfate-67Ga complex
Shinya Abe1, Yositaka Shimizu, Junko Ohtsuki
1Department of Radiopharmacy, Tohoku Pharmaceutical University, Sendai, Japan. shin-abe@tohoku-pharm.ac.jp
Abstract:
We attempted to identify the exchange mechanism of transferrin-67Ga complex to heparan sulfate-67Ga complex. The effect of phosphate on the binding ability of 67Ga to transferrin and heparan sulfate was studied by the dialysis method. The phosphate inhibited the binding ability of 67Ga to transferrin, while the phosphate enhanced the 67Ga binding ability to heparan sulfate. The results suggest that the phosphate is involved in the translocation of 67Ga from the transferrin to the heparan sulfate.
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