Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Manganese oxidation state and its implications for toxicity.

Stephen H Reaney1, Catherine L Kwik-Uribe, Donald R Smith

  • 1Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA. stevereaney@hotmail.com

Chemical Research in Toxicology
|September 17, 2002
PubMed
Summary

Manganese oxidation state impacts cellular uptake and toxicity. Exposure to manganese(III) significantly increased cellular manganese accumulation and inhibited aconitase activity more than manganese(II).

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neuron-specific modulation of SLC30A10 identifies dopaminergic and glutamatergic neurons as targets of manganese-induced motor disease.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Oral guanfacine treatment ameliorates the ADHD-like symptoms caused by developmental manganese exposure.

Progress in neuro-psychopharmacology & biological psychiatry·2025
Same author

NR4A1 is a HIF-dependent repressor of SLC30A10 transcription that controls manganese homeostasis.

The Journal of biological chemistry·2025
Same author

Elevated brain manganese induces motor disease by upregulating the kynurenine pathway of tryptophan metabolism.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Sex-specific associations of a ferroalloy metal mixture with motor function in Italian adolescents.

Environmental epidemiology (Philadelphia, Pa.)·2024
Same author

PHD2 enzyme is an intracellular manganese sensor that initiates the homeostatic response against elevated manganese.

Proceedings of the National Academy of Sciences of the United States of America·2024

Area of Science:

  • Environmental toxicology
  • Biochemistry
  • Inorganic chemistry

Background:

  • Manganese (Mn) is essential for mammalian development but toxic at high levels.
  • Mn's biological functions depend on its oxidation state (e.g., Mn(II), Mn(III)).
  • The impact of Mn oxidation state on cellular uptake and toxicity is poorly understood.

Purpose of the Study:

  • Investigate how Mn oxidation state affects cellular uptake and toxicity.
  • Examine the dynamics of EPR-measurable Mn(II) in plasma and cell lysates.
  • Assess the effects of Mn(II) versus Mn(III) exposure on cellular aconitase activity and Mn uptake.

Main Methods:

  • Exposed fresh human plasma and PC12 cell lysates to manganese(II) chloride and manganese(III) pyrophosphate.
  • Measured EPR-detectable Mn(II) dynamics.

Related Experiment Videos

  • Assessed total cellular aconitase activity and cellular Mn uptake in intact PC12 cells.

    • Main Results:

    • In vitro exposure of plasma/lysates to Mn(II) or Mn(III) yielded similar EPR-measurable Mn(II).
    • Mn(III) exposure significantly inhibited cellular aconitase activity more than Mn(II).
    • Intact PC12 cells accumulated significantly more Mn when exposed to Mn(III) compared to Mn(II).

    Conclusions:

    • Mn speciation is dynamic in biological systems.
    • The oxidation state of manganese is crucial in mediating cellular toxicity.
    • Elevated environmental exposure to various Mn oxidation states warrants concern.