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Protective effects of Ganoderma lucidum polysaccharides peptide on injury of macrophages induced by reactive oxygen
1Department of Pharmacology, School of Basic Medical Science, Peking University Health Science Center, Beijing 100083, China.
Aim:
To study the protective effects of Ganoderma lucidum polysaccharides peptide (GLPP) on the mice peritoneal macrophages injured by reactive oxygen species (ROS), derived from tert-butylhydroperoxide (tBOOH) in vitro and in vivo.
Methods:
Mice peritoneal macrophages were injured by ROS, derived from tBOOH. The survival rate of macrophages was measured by MTT assay, and the morphological changes of macrophages were observed under light and electron microscopes.
Results:
GLPP (50, 100, 200 mg/kg, ip for 5 d) could inhibit the foam cell formation and necrosis of macrophages. The survival rate of macrophages was increased. GLPP (3.125, 12.5, 50, 200 mg/L) given to the cultured macrophages brought the same protective effects. Under the electron microscope it was found that GLPP (100 mg/kg, ip, for 5 d) could protect the organelle such as mitochondria against injury by tBOOH.
Conclusion:
GLPP had significant scavenging ROS and antioxidant effects.
Insights
Ganoderma lucidum polysaccharides peptide (GLPP) protects mice macrophages from oxidative stress. This study demonstrates GLPP’s significant antioxidant and reactive oxygen species (ROS) scavenging capabilities, enhancing cell survival.
Area of Science:
- Immunology
- Pharmacology
- Biochemistry
Background:
- Oxidative stress from reactive oxygen species (ROS) damages cells, including macrophages.
- tert-butylhydroperoxide (tBOOH) is a common inducer of ROS-mediated cellular injury.
- Ganoderma lucidum polysaccharides peptide (GLPP) is a potential therapeutic agent.
Purpose of the Study:
- To investigate the protective effects of GLPP against ROS-induced injury in mice peritoneal macrophages.
- To evaluate GLPP's efficacy both in vitro and in vivo.
Main Methods:
- Mice peritoneal macrophages were exposed to ROS generated by tBOOH.
- Cell viability was assessed using MTT assay.
- Morphological changes were observed via light and electron microscopy.
Main Results:
- GLPP treatment inhibited foam cell formation and macrophage necrosis.
- Macrophage survival rates significantly increased following GLPP administration.
- Electron microscopy revealed GLPP protects organelles, particularly mitochondria, from tBOOH-induced damage.
Conclusions:
- GLPP exhibits significant ROS scavenging activity.
- GLPP demonstrates potent antioxidant effects, protecting macrophages from oxidative injury.
