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DNA Replication-Dependent Histone H2A mRNA Expression in Pea Root Tips.

E. Y. Tanimoto¹, T. L. Rost, L. Comai

¹Section of Plant Biology, Division of Biological Sciences, University of California, Davis, California 95616-8537 (E.Y.T., T.L.R.).

Plant Physiology

|December 1, 1993

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Pea histone H2A mRNA expression is linked to DNA replication. This study shows H2A mRNA accumulation primarily occurs during the S phase of the cell cycle in Pisum sativum root cells.

Area of Science:

Plant Molecular Biology
Cell Biology
Genetics

Background:

Histone H2A mRNA exhibits selective expression in specific cell populations within the pea (Pisum sativum) root apical meristem.
The cell cycle regulation of histone gene expression is crucial for DNA replication and cell division.

Purpose of the Study:

To investigate the association between histone H2A mRNA expression and the cell cycle, specifically DNA replication (S phase).
To determine if H2A mRNA accumulation is replication-dependent in Pisum sativum root cells.

Main Methods:

Utilized a double-labeling technique combining [3H]thymidine incorporation and autoradiography to identify S-phase cells.
Employed in situ hybridization with digoxigenin-labeled probes to detect H2A mRNA localization.

Related Experiment Videos

Assessed the effect of inhibiting DNA synthesis with hydroxyurea on H2A mRNA levels.

Main Results:

A significant overlap was observed: approximately 92% of S-phase cells expressed H2A mRNA, and 85% of H2A mRNA-expressing cells were in S phase.
Synchronous, co-located expression of H2A mRNA and S-phase cells was found in scattered proliferating cell packets basal to the promeristem.
Inhibition of DNA synthesis by hydroxyurea led to a specific decrease in steady-state H2A mRNA levels.
Neither H2A mRNA nor S-phase cells were detected in the quiescent center or mature root cap.

Conclusions:

Cell-specific expression of pea histone H2A mRNA is dependent on DNA replication.
Histone H2A mRNA is transiently accumulated during the S phase of the cell cycle.
H2A mRNA accumulation likely begins in late G1 and ceases in late S, coinciding with DNA replication.