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Nutrient Uptake Changes in Ascorbate Free Radical-Stimulated Onion Roots.

J. A. Gonzalez-Reyes1, A. Hidalgo, J. A. Caler

  • 1Departamento de Biologia Celular, Universidad de Cordoba, 14004 Cordoba, Spain.

Plant Physiology
|January 1, 1994
PubMed
Summary

Ascorbate free radical stimulates nutrient uptake and root growth in onion (Allium cepa) by increasing vacuole size. This suggests a redox system activation enhances nutrient absorption and elongation.

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Purification and Characterization of Two Distinct NAD(P)H Dehydrogenases from Onion (Allium cepa L.) Root Plasma Membrane.

Plant physiology·1994

Area of Science:

  • Plant Physiology
  • Molecular Biology
  • Biochemistry

Background:

  • Nutrient uptake is crucial for plant growth and development.
  • Ascorbate free radical's role in plant nutrient transport is not fully understood.
  • Allium cepa (onion) is a model organism for studying root physiology.

Purpose of the Study:

  • To investigate the effect of ascorbate free radical on nutrient uptake in Allium cepa roots.
  • To elucidate the relationship between vacuole dynamics and nutrient absorption.
  • To explore the involvement of transplasma membrane redox systems.

Main Methods:

  • Long-term treatment of Allium cepa roots with ascorbate free radical.
  • Measurement of glucose, fucose, sucrose, and nitrate uptake kinetics.

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  • Analysis of vacuole volume and tonoplast surface area.
  • Assessment of root elongation.

    • Main Results:

    • Ascorbate free radical treatment stimulated glucose and fucose uptake, altering kinetics from saturation to linear.
    • Nitrate and sucrose uptake rates increased, but kinetics remained similar to controls.
    • Pretreatment with ascorbate free radical enhanced uptake rates over 48 hours.
    • Increased vacuole volume and tonoplast surface area correlated with stimulated nutrient uptake and root elongation.

    Conclusions:

    • Ascorbate free radical enhances nutrient uptake and root elongation in Allium cepa.
    • Increased vacuolar volume is linked to stimulated nutrient absorption.
    • Activation of a transplasma membrane redox system by ascorbate free radical is implicated in these responses.