Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 25, 2026

Laboratory Production of Biofuels and Biochemicals from a Rapeseed Oil through Catalytic Cracking Conversion

Laboratory Production of Biofuels and Biochemicals from a Rapeseed Oil through Catalytic Cracking Conversion

Published on: September 2, 2016

High-Oleate Oilseeds Fail to Develop at Low Temperature.

M. F. Miquel¹, J. A. Browse

¹Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340.

Plant Physiology

|October 1, 1994

Summary

This summary is machine-generated.

Related Concept Videos

Seed Structure and Early Development of the Sporophyte

Seed Structure and Early Development of the Sporophyte

Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo.

Responses to Heat and Cold Stress

Responses to Heat and Cold Stress

Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.

Structure of Lipids

Structure of Lipids

Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic birds and...

Biofuels

Biofuels

The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Intracellular Levels of Free Linolenic and Linoleic Acids Increase in Tomato Leaves in Response to Wounding.

Plant physiology·1996

Same journal

CYSTEINE-RICH RLK2 regulates development via callose synthase-dependent symplastic transport in Arabidopsis.

Plant physiology·2026

Same journal

H2O2 oxidation of VvMYB APL reduces VvHSP20-43 expression and promotes grape ripening.

Plant physiology·2026

Same journal

Mitigating Constraints in Harvest Index and Yield of Densified Populations via Sink Modulation of Narrowing Pollination Time Gaps within Maize Ear.

Plant physiology·2026

Same journal

The MrHY5-mru-miR396-MrGRF4 module regulates UV-B-induced quercetin biosynthesis in Chinese bayberry (Morella rubra cv. Biqi).

Plant physiology·2026

Same journal

The transcription factor StC3H14 enhances cold tolerance through the CBF-dependent pathway in potato.

Plant physiology·2026

Same journal

Jasmonic acid and PpeMYC2 regulate peach fruit ripening by controlling polyamine levels and anthocyanin biosynthesis.

Plant physiology·2026

See all related articles

Genetic modification of oilseed crops to increase oleate may reduce performance. Fad2 mutants showed reduced viability and slower germination at low temperatures, indicating potential field issues for high-oleate varieties.

Area of Science:

Plant Biology
Biochemistry
Agricultural Science

Background:

The fad2 mutant in Arabidopsis thaliana lacks endoplasmic reticulum oleate desaturase activity.
This enzyme is crucial for synthesizing polyunsaturated lipids in developing oil crop seeds.
The fad2 mutant serves as a model for genetically engineered high-oleate oilseeds like soybean and canola.

Purpose of the Study:

To investigate the impact of reduced oleate desaturase activity on seed viability and germination.
To assess the performance of fad2 mutants under varying temperature conditions.
To evaluate the implications of genetic manipulation for high-oleate oilseed crop performance.

Main Methods:

Comparative analysis of wild-type and fad2 mutant Arabidopsis seeds.

More Related Videos

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

Published on: January 11, 2017

Ultrasonic-Assisted Preparation of Biodiesel Products from Vegetable Oils

Ultrasonic-Assisted Preparation of Biodiesel Products from Vegetable Oils

Published on: April 19, 2024

Related Experiment Videos

Last Updated: Jun 25, 2026

Laboratory Production of Biofuels and Biochemicals from a Rapeseed Oil through Catalytic Cracking Conversion

Laboratory Production of Biofuels and Biochemicals from a Rapeseed Oil through Catalytic Cracking Conversion

Published on: September 2, 2016

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

Published on: January 11, 2017

Ultrasonic-Assisted Preparation of Biodiesel Products from Vegetable Oils

Ultrasonic-Assisted Preparation of Biodiesel Products from Vegetable Oils

Published on: April 19, 2024

Controlled temperature treatments (22°C and 6°C) during seed development and germination.

Assessment of seed viability, lipid content, and germination rates.

Main Results:

Fad2 mutant seeds exhibited high viability at normal temperatures (22°C) but significantly declined germination at low temperatures (6°C).
Low-temperature exposure during late seed development also reduced fad2 mutant germination.
Fad2 seeds produced at 22°C had lower lipid content and slower germination at 10°C and 6°C compared to wild-type.

Conclusions:

Molecular genetic manipulation to increase oleate levels in oilseeds may compromise field performance.
Reduced oleate desaturase activity can lead to temperature-sensitive seed viability and germination.
Findings suggest potential challenges for high-oleate oilseed varieties in certain environmental conditions.