Interaction between the Anion Exchange Protein and the Glucose Transport Protein in Human Red Cell Membranes
Zhi-Hong Zhang1, Feng Peng, Xiao-Quan Li
1Department of Physiology and Biophysics, Fudan University, Shanghai 200433, China.
Abstract:
Cytochalasin B was used to inhibit the glucose transport through the glucose transport protein (GluT-1) in red cell membranes. It was observed that the anion transport was increased at the same time. The rate of anion transport was also increased when GluT-1 was bound with its substrate molecule-glucose. On the other hand, the experimental result on the relationship between glucose transport and Cl(-) concentration showed that K(m) was decreased and V(max) increased following the increase of Cl(-) concentration. There would be a bidirectional linkage between Band 3 (anion exchange protein) and GluT-1 in the intact red cell membranes. In other words, the conformation change of membrane protein could affect the function of other kinds of membrane proteins.
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