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The Study of a New Type of Membrane for Biosensor
Shun-Guang Wang1, Xin-Song Ji, Zhong-Yi Yuan
1Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031, China.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica Et Biophysica Sinica
|January 1, 1996
Abstract:
Polyvinyl alcohol (PVA) was selected to develop a new type of membrane for biosensor. The PVA membrane was first activated by cyanogens bromide, followed by coupling with ethyl diamine. After treated by glutaraldehyde, glucose oxidase and lactate oxidase were immobilized onto the membrane respectively. Coupled with oxygen electrode, the enzyme membrane was used to construct glucose and lactate biosensor respectively. The linear range was 5 mg/dl-800 mg/dl for glucose and 1mg/dl-35 mg/dl for lactate, and the response time of these biosensors was 10 seconds.