Related Experiment Video
Updated: Aug 17, 2026
Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
Published on: June 17, 2015
DNA microarray analysis of differentially expressed genes responsive to bisphenol A, an alkylphenol derivative, in an
Yoshiaki Tabuchi1, Qing-Li Zhao, Takashi Kondo
1Molecular Genetics Research Center, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Sugitani, Japan. ytabu@ms.toyama-mpu.ac.jp
Abstract:
To identify genes elicited by bisphenol A (BPA) in Sertoli cells, we carried out a microarray analysis of TTE3 cells (a mouse Sertoli cell line) treated with BPA. BPA (100, 200 and 400 microM) induced cell death concentration-dependently, with levels being 25%, 33% and 96%, respectively. Of the 1,081 genes analyzed, 3 genes showed decreased levels of expression while the remaining 10 genes showed increased levels in the cells treated with a subtoxic dose of BPA (200 microM). The expressions of six genes were confirmed by the TaqMan assay. These findings suggest that DNA microarray analysis is a useful tool for investigating the molecular mechanisms of the toxic effects of BPA in testicular cells.
More Related Videos
08:28Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays
Published on: April 26, 2018
07:08Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants
Published on: March 6, 2018