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Related Experiment Videos

An arsenic(III)-oxidizing bacterial population: selection, characterization, and performance in reactors.

F Battaglia-Brunet1, M-C Dictor, F Garrido

  • 1BRGM, Environment & Process Division, Biotechnology Unit, Orléans, France. f.battaglia@brgm.fr

Journal of Applied Microbiology
|September 18, 2002
PubMed
Summary

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A novel bacterial population, CASO1, was selected for autotrophic arsenic(III) oxidation from a mining environment. This consortium demonstrates high efficiency in oxidizing arsenite without organic nutrients, offering potential for arsenic bioremediation.

Area of Science:

  • Environmental Microbiology
  • Bioremediation
  • Arsenic Biogeochemistry

Background:

  • Arsenic contamination poses significant environmental and health risks.
  • Biological oxidation of arsenic(III) (arsenite) is a crucial step in arsenic remediation.
  • Autotrophic microbial processes are desirable for sustainable bioremediation strategies.

Purpose of the Study:

  • To isolate and characterize an autotrophic arsenic(III)-oxidizing bacterial population (CASO1).
  • To evaluate the performance of CASO1 in bioreactors for arsenic oxidation.
  • To identify the microbial composition of the selected consortium.

Main Methods:

  • Enrichment and selection of CASO1 using an arsenic(III)-containing medium without organic substrates.
  • Performance evaluation under batch and continuous culture conditions.

Related Experiment Videos

  • Molecular biology techniques (16S rDNA sequencing) for microbial identification.

    • Main Results:

    • CASO1 exhibited significant arsenic(III) oxidation activity across a pH range of 3-8, with optimal temperature at 25°C.
    • The consortium demonstrated high tolerance to arsenic(III), with oxidation observed at concentrations up to 1000 mg/L.
    • Continuous culture achieved an arsenic(III) oxidation rate of 160 mg/L/h. The consortium comprises strains related to Ralstonia picketii and Thiomonas, with one strain potentially representing a new species.

    Conclusions:

    • Autotrophic arsenic(III) oxidation by microbial consortia is feasible and highly efficient.
    • The CASO1 population shows promise for the biological treatment of arsenic-contaminated waste and groundwater.
    • The identification of novel or specialized microorganisms contributes to the field of bioremediation.