Related Experiment Video

Updated: Sep 29, 2026

Single-Molecule FRET Imaging for Observing the Conformational Dynamics of Dynamin-Like GTPase Atlastin
10:19

Single-Molecule FRET Imaging for Observing the Conformational Dynamics of Dynamin-Like GTPase Atlastin

Published on: January 24, 2025

Molecular and functional interaction of the ATP-binding cassette transporter A1 with Fas-associated death domain

Christa Buechler1, Salim Maa Bared, Charalampos Aslanidis

  • 1Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany.

The Journal of Biological Chemistry
|September 18, 2002
PubMed

Insights

The ATP-binding cassette transporter A1 (ABCA1) requires binding to other proteins, like Fas-associated death domain protein (FADD), to effectively regulate cholesterol and phospholipid homeostasis.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • ATP-binding cassette transporter A1 (ABCA1) is crucial for cellular lipid homeostasis.
  • ABCA1's precise function and regulatory mechanisms, including protein interactions, are not fully understood.

Purpose of the Study:

  • To identify proteins that interact with ABCA1.
  • To investigate the functional significance of ABCA1-interacting proteins in lipid transport.

Main Methods:

  • Screening of a human liver yeast two-hybrid library using the C-terminal domain of ABCA1.
  • Confirmation of protein interactions using pull-down assays and co-immunoprecipitation.
  • Functional assays in HepG2 cells involving recombinant FADD or ABCA1 C-terminus.

Main Results:

  • Fas-associated death domain protein (FADD) was identified as an ABCA1-interacting protein.
  • The ABCA1-FADD interaction was validated through biochemical assays.
  • Inhibition of FADD or ABCA1 C-terminus impaired phospholipid transfer to apoA-I in HepG2 cells.

Conclusions:

  • ABCA1 function in phospholipid transport is dependent on interactions with other proteins, including FADD.
  • This study reveals a novel connection between high-density lipoprotein (HDL) metabolism and FADD, a protein typically involved in death receptor signaling.

More Related Videos

F1FO ATPase Vesicle Preparation and Technique for Performing Patch Clamp Recordings of Submitochondrial Vesicle Membranes08:21

F1FO ATPase Vesicle Preparation and Technique for Performing Patch Clamp Recordings of Submitochondrial Vesicle Membranes

Published on: May 4, 2013

Measuring Composition of CD95 Death-Inducing Signaling Complex and Processing of Procaspase-8 in this Complex07:17

Measuring Composition of CD95 Death-Inducing Signaling Complex and Processing of Procaspase-8 in this Complex

Published on: August 2, 2021

Related Concept Videos

The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
The ADP/ATP Carrier Protein01:42

The ADP/ATP Carrier Protein

ADP/ATP carrier or AAC protein is the most abundant carrier protein in the inner mitochondrial membrane. It transports large quantities of ADP and ATP, equivalent to the average human body weight, every day. Among other transporters, ACC protein is one of the best-studied members of the mitochondrial carrier protein family. The ADP/ATP carrier protein comprises two transmembrane helices connected to a loop and a single alpha-helix on the matrix side. It switches between two conformational...
ATP Synthase: Mechanism01:48

ATP Synthase: Mechanism

In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased ATP...
Membrane Asymmetry Regulating Transporters01:19

Membrane Asymmetry Regulating Transporters

Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...