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Related Experiment Video

Updated: Jan 19, 2026

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Published on: December 8, 2023

An aptamer-based quartz crystal protein biosensor.

Michael Liss¹, Birgit Petersen, Hans Wolf

¹Institute of Medical Microbiology and Hygiene, University of Regensburg, Germany.

Analytical Chemistry

|September 19, 2002

Summary

This summary is machine-generated.

We created a novel biosensor using DNA aptamers to detect proteins like human IgE in real time. This aptamer biosensor offers high sensitivity, stability, and a wider detection range compared to traditional antibody methods.

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Sample Preparation in Quartz Crystal Microbalance Measurements of Protein Adsorption and Polymer Mechanics

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Published on: January 22, 2020

Area of Science:

Biotechnology
Biosensor Technology
Molecular Diagnostics

Background:

Accurate protein detection is crucial for diagnostics and research.
Existing biosensors often rely on antibodies, which can have limitations in stability and cost.
Developing alternative recognition elements for biosensors is an active area of research.

Purpose of the Study:

To develop and validate a quartz crystal biosensor for real-time detection of unlabeled proteins.
To compare the performance of DNA aptamers with traditional antibodies as ligands in a biosensor.
To assess the stability, sensitivity, and detection range of an aptamer-based biosensor.

Main Methods:

Development of a quartz crystal biosensor.
Immobilization of single-stranded DNA aptamers and anti-IgE antibodies as ligands.
Detection of human immunoglobulin E (IgE) as an analyte.
Real-time measurement of protein concentrations and ligand affinity.
Evaluation of biosensor regeneration and stability.

Main Results:

Aptamers demonstrated equivalent specificity and sensitivity to antibodies for IgE detection (0.5 nmol/L).
The aptamer-based biosensor exhibited tolerance to repeated regeneration cycles with minimal loss of sensitivity.
Dense, well-oriented aptamer immobilization extended the linear detection range by 10-fold.
Aptamer-ligand complexes showed heat resistance and stability over several weeks in complex protein mixtures.

Conclusions:

DNA aptamers are effective and robust ligands for biosensor development, matching antibody performance.
Aptamer-based biosensors offer advantages in stability, detection range, and immobilization chemistry.
This technology holds promise for quantitative protein analysis in complex biological samples and proteomic studies.