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Published on: April 29, 2017
[Fast method of labelling leukocytes with 99mTc HM PAO]
C Piera1, A Vázquez, M A Hernández
1Servei de Medicina Nuclear, Centre de Diagnòstic per la Imatge, IDIBAPS, Hospital Clínic, Barcelona, Spain. cpiera@clinic.ub.es
Revista Espanola De Medicina Nuclear
|September 19, 2002
Summary
A new centrifugation method significantly reduces leukocyte labelling time with 99mTc HM-PAO. This method offers a valuable alternative for nuclear medicine services without compromising scintigraphic image quality.
Area of Science:
- Nuclear Medicine
- Radiopharmacy
- Hematology
Background:
- Leukocyte labelling with 99mTc HM-PAO is crucial for infection imaging.
- Traditional sedimentation methods are time-consuming (30-90 minutes).
- Optimizing leukocyte-rich plasma (LRP) preparation is key for efficient labelling.
Purpose of the Study:
- To develop a faster method for obtaining LRP using low-gravity centrifugation.
- To compare the efficiency and purity of LRP obtained by centrifugation versus sedimentation.
- To evaluate the impact of the new method on leukocyte labelling yield and scintigraphic imaging.
Main Methods:
- Determined optimal centrifugation speed (g-force) for leukocyte recovery.
- Analyzed cellular composition of LRP from centrifugation and sedimentation.
- Compared leukocyte labelling results between the traditional sedimentation method (SM) and the proposed centrifugation method (CM) in two patient groups.
Main Results:
- Centrifugation at 5xg yielded the highest leukocyte recovery (93.1%).
- Leukocyte recovery was similar between centrifugation and sedimentation methods.
- Centrifugation resulted in greater red blood cell contamination but higher labelling yield and radiochemical purity.
- Scintigraphic images showed no significant difference between methods due to low red blood cell radiopharmaceutical uptake.
Conclusions:
- The proposed centrifugation method (CM) significantly reduces leukocyte labelling time with 99mTc HM-PAO.
- CM provides an effective labelling alternative for nuclear medicine services.
- The quality of scintigraphic images is not affected by the centrifugation method.
