Related Experiment Video

Updated: Sep 29, 2026

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

Antigenic relationships between six genotypes of the small hydrophobic protein gene of mumps virus

Claes Örvell1, Tesfaldet Tecle1, Bo Johansson1

  • 1Huddinge University Hospital, Department of Clinical Virology, Karolinska Institutet, SE-141 86 Stockholm, Sweden1.

The Journal of General Virology
|September 19, 2002
PubMed

Insights

Mumps virus genotypes C, D, G, H, and I are antigenically indistinguishable. However, genotype A comprises three distinct serotypes, indicating potential challenges for mumps virus vaccines in providing broad protection.

Area of Science:

  • Virology
  • Immunology
  • Molecular Biology

Background:

  • Mumps virus exhibits genetic diversity with multiple genotypes identified.
  • Antigenic characterization is crucial for understanding mumps virus evolution and vaccine efficacy.

Purpose of the Study:

  • To compare the antigenicity of six mumps virus genotypes (A, C, D, G, H, I).
  • To investigate the antigenic relationship between different strains within genotype A.
  • To assess the cross-neutralization capacity of vaccines against endemic strains.

Main Methods:

  • Immunofluorescence assays using monoclonal antibodies against the haemagglutinin-neuraminidase protein.
  • Cross-neutralization tests with human post-vaccination sera and rabbit hyperimmune sera.
  • Genotyping based on the small hydrophobic protein gene sequence.

Main Results:

  • Mumps virus genotypes C, D, G, H, and I could not be antigenically differentiated.
  • Three distinct serotypes were identified within genotype A: SBL-1, Jeryl Lynn (JL), and Kilham strains.
  • Vaccination with the JL strain showed lower neutralization titers against the endemic SBL-1 strain compared to homologous titers.

Conclusions:

  • Mumps virus genotypes C, D, G, H, and I represent a single serological group.
  • Genotype A contains at least three distinct serotypes, suggesting limited cross-protection.
  • Current mumps virus vaccines may offer suboptimal protection against circulating endemic strains like SBL-1.

More Related Videos

Co-immunoprecipitation of the Mouse Mx1 Protein with the Influenza A Virus Nucleoprotein09:39

Co-immunoprecipitation of the Mouse Mx1 Protein with the Influenza A Virus Nucleoprotein

Published on: April 21, 2015

Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes08:52

Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes

Published on: July 26, 2019

Related Concept Videos

Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
Cross-reactivity00:42

Cross-reactivity

Overview