Studies on the Effect of Recombinant Human Tumor Necrosis Factor-alpha on Leukemic Cell Lines by (31)P-NMR

31P nuclear magnetic resonance (NMR) spectroscopy has been used for monitoring cellular metabolism in human lymphoma cell line Molt-4 and human promyelocyte leukemia cell line HL-60. (31)P-NMR spectra of Molt-4 cells and HL-60 cells consist primarily of peaks of phosphomonoesters, inorganic phosphate (Pi), diphosphodiesters and ATP. A decrease in the ATP/Pi ration occurred after treatment of the Molt-4 cells with recombinant human tumor necrosis factor-alpha(rhTHF-alpha). The ATP decreased after treatment of the Hl-60 cells with rhTNF-alpha.