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Preparation of Neoglycoproteins by Using Homo-bifunctional Reagent as the Coupling Agent
1Cancer Hospital, Shanghai Medical University, Shanghai 200032, China.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica Et Biophysica Sinica
|January 1, 1996
Abstract:
Several neoglycoproteins (Man-BSA, Gal-BSA, Glc-BSA, Lac-BSA, GlcNAc-BSA, Fuc-BSA and Rha-BSA) have been prepared by using homo-bifunctional reagents (divinyl sulfone, DVS and 1,4-butanediol diglycidyl ether, Epoxy) as coupling agents and purilied with the respective immobilized lectins (except Fuc-BSA and Rha-BSA). These purified neoglycoproteins showed binding specificity to the corresponding lectins by hemagglutinating inhibition test and double diffusion experiment.