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Bioactive phenolic derivatives from Acaena splendens methanol extract
N Backhouse1, C Delporte, R Negrete
1Pharmacognosy Laboratory, Faculty of Chemical and Pharmaceutical Sciences, University of Chile, P.O. Box 233, Santiago 1, Chile.
Phytotherapy Research : PTR
|September 19, 2002
Summary
Acaena splendens demonstrated significant fever reduction and anti-inflammatory effects in animal models. Flavonoids isolated from the plant showed potent antipyretic and anti-inflammatory activities, validating traditional medicinal uses.
Area of Science:
- Pharmacology
- Ethnobotany
- Natural Product Chemistry
Background:
- Acaena splendens is utilized in Chilean folk medicine for treating fever and inflammation.
- Scientific validation of its traditional uses is essential for understanding its therapeutic potential.
Purpose of the Study:
- To investigate the in vivo antipyretic and anti-inflammatory properties of Acaena splendens extracts.
- To identify the active compounds responsible for these pharmacological effects.
Main Methods:
- In vivo studies using bacterial pyrogen-induced fever in rabbits and carrageenan-induced paw edema in guinea pigs.
- Sequential extraction and column chromatography for isolation and purification of active compounds.
- Bioassay-guided fractionation to identify specific phytochemicals.
Main Results:
- The methanol extract (ME-1) exhibited significant antipyretic activity (45.7%).
- Column fractionation yielded a flavonoid-rich mixture (C4) with potent anti-inflammatory (55.7%) and antipyretic (98.9%) effects at 600 mg/kg.
- Active compounds identified include (-,-)-epicatechin, tiliroside, 7-O-acetyl-3-O-beta-D-glucosyl-kaempferol, and 7-beta-D-glucosyloxy-5-hydroxy-chromone.
Conclusions:
- Acaena splendens possesses significant in vivo antipyretic and anti-inflammatory activities.
- Flavonoids are key contributors to the plant's medicinal properties.
- The study supports the traditional use of Acaena splendens for fever and inflammation management.