Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tardive decrease of astrocytic glutamate transporter protein in transgenic mice with ALS-linked mutant SOD1.

H Warita1, Y Manabe, T Murakami

  • 1Department of Neurology, Graduate School of Medicine and Dentistry, Okayama University, Japan.

Neurological Research
|September 20, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immunomodulatory potential of particular Trichinella spiralis muscle larvae excretory-secretory components.

International journal for parasitology·2016
Same author

Differential immunological responses induced by infection with female muscle larvae and newborn larvae of Trichinella pseudospiralis.

Veterinary parasitology·2013
Same author

Trichinella: what is going on during nurse cell formation?

Veterinary parasitology·2013
Same author

Minimally required heat doses for various tumour sizes in induction heating cancer therapy determined by computer simulation using experimental data.

International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·2010
Same author

Hypoglycaemia induced by Trichinella infection is due to the increase of glucose uptake in infected muscle cells.

International journal for parasitology·2008
Same author

Genetic relationships among Trichinella pseudospiralis isolates from Australian, Nearctic, and Palearctic regions.

Parasitology research·2007
Same journal

Predictive ability of cardiac biomarkers for early risk stratification and 3-month functional outcomes after reperfusion therapy in acute ischemic stroke.

Neurological research·2026
Same journal

Luteolin reduces sciatic nerve damage and modulates TRPV1 and TRPM2 expression in diabetic rats.

Neurological research·2026
Same journal

Cholinergic regulation of memory retrieval: scopolamine reduces hippocampal neurotrophic and metabolic support.

Neurological research·2026
Same journal

Effect of intravenous thrombolysis on arterial and venous profiles in large-vessel occlusion stroke: a retrospective propensity score-matched study.

Neurological research·2026
Same journal

Association between post-treatment neuroimaging markers of cerebral small vessel disease and short-term efficacy of recombinant tissue plasminogen activator in acute stroke patients.

Neurological research·2026
Same journal

Association between non-high-density lipoprotein cholesterol to high-density lipoprotein cholesterol ratio (NHHR) and the characteristics of carotid artery plaques: a retrospective study and machine learning model construction.

Neurological research·2026
See all related articles

Glutamate transporter EAAT2 levels remain stable during early paralysis in SOD1 mice, suggesting it is not a primary factor in motor neuron death. EAAT2 and GFAP show distinct expression patterns in the spinal cord.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by motor neuron loss.
  • Mutations in superoxide dismutase 1 (SOD1) are a known cause of familial ALS.
  • Glutamate excitotoxicity, mediated by impaired glutamate transport, is implicated in ALS pathogenesis.

Purpose of the Study:

  • To investigate the expression patterns of glutamate transporter proteins EAAT2 and EAAT3 in the spinal cord of a mouse model of ALS.
  • To examine the relationship between EAAT2 and GFAP expression in the ventral horn during disease progression.
  • To determine the role of EAAT2 in motoneuron degeneration in the G93A SOD1 mouse model.

Main Methods:

  • Immunocytochemistry was used to examine the expression of glutamate transporter proteins (EAAT2, EAAT3) and glial fibrillary acidic protein (GFAP) in the spinal cord of G93A SOD1 transgenic mice.

Related Experiment Videos

  • Expression levels were analyzed at different stages of the disease, including pre-symptomatic, paralytic, and terminal stages.

    • Main Results:

    • Astroglial EAAT2 protein levels were maintained in the ventral horn until the terminal stage of the disease, despite the onset of paralysis.
    • Neuronal EAAT3 protein levels decreased concurrently with EAAT2 at the terminal stage.
    • Glial fibrillary acidic protein (GFAP) immunoreactivity showed a progressive increase from 25 weeks of age, indicating reactive astrogliosis.
    • Dissociative expression patterns between EAAT2 and GFAP were observed in the ventral horn.

    Conclusions:

    • The findings suggest that EAAT2 is not an early or primary contributor to motoneuron death in the G93A SOD1 mouse model.
    • The observed dissociation between EAAT2 and GFAP expression highlights complex glial responses in ALS.
    • Further research is needed to elucidate the precise mechanisms of motoneuron degeneration in this ALS model.