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Related Experiment Videos

A Single Gene May Encode Differentially Localized Ca2+-ATPases in Tomato.

N. Ferrol1, A. B. Bennett

  • 1Mann Laboratory, Department of Vegetable Crops, University of California at Davis, Davis, California 95616.

The Plant Cell
|July 1, 1996
PubMed
Summary

A single tomato gene encodes two Ca2+-ATPase isoforms, localized to vacuolar and plasma membranes. These isoforms exhibit similar vanadate-sensitive ATP-dependent Ca2+ transport activities, suggesting a shared functional role.

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Plant physiology·1993

Area of Science:

  • Plant Molecular Biology
  • Cellular Physiology
  • Biochemistry

Background:

  • A sarcoplasmic reticulum-type Ca2+-ATPase (LCA) was previously identified in tomato.
  • The subcellular localization and function of LCA were not fully understood.

Purpose of the Study:

  • To determine the subcellular localization of the Lycopersicon Ca2+-ATPase (LCA).
  • To investigate the functional properties of LCA in different cellular compartments.

Main Methods:

  • Generation of specific polyclonal antibodies against an LCA fusion protein.
  • Immunodetection of LCA polypeptides in tomato root membranes.
  • Assay of ATP-dependent Ca2+ transport activities in isolated membrane fractions.

Main Results:

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  • Antibodies recognized two polypeptides (116 and 120 kD) in vacuolar and plasma membranes.
  • Vanadate-sensitive Ca2+ transport activity correlated with LCA protein distribution.
  • Both tonoplast and plasma membrane Ca2+-ATPase activities showed similar inhibition profiles and were affected by LCA antibodies.

Conclusions:

  • A single tomato gene (LCA) likely encodes two P-type Ca2+-ATPase isoforms.
  • These isoforms are differentially localized to the tonoplast and plasma membrane.
  • The LCA isoforms may play distinct but related roles in calcium transport in tomato roots.