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Highly efficient oligosaccharide synthesis on water-soluble polymeric primers by recombinant glycosyltransferases
S Nishiguchi1, K Yamada, Y Fuji
1Sapporo Laboratory ofr Glycocluster Project, Japan Bioindustry Association, Sapporo 060-0810, Japan.
Chemical Communications (Cambridge, England)
|September 21, 2002
Abstract:
Recombinant beta-1,4-galactosyltranferase (beta 1,4-GalT) and alpha-2,6-sialytransferase (alpha 2,6-SiaT) immobilised covalently with activated Sepharose beads were employed for the practical synthesis of a trisaccharide derivative, Neu-5Ac alpha(2-->6)Gal beta(1-->4)GlcNAc beta-O-(CH2)6-NH2, on a water-soluble primer having GlcNAc residues through a alpha-chymotrypsin-sensitive linker.