A glucose oxidase inmobilized electrode based on modified graphite

Area of Science:

• Electrochemistry
• Biotechnology
• Biosensors

Background:

• Enzyme immobilization is crucial for developing stable and sensitive biosensors.
• Glucose oxidase is a key enzyme for glucose detection, but its stability and reusability are challenges.
• Electrochemical modification of electrode surfaces enhances enzyme immobilization and sensor performance.

Purpose of the Study:

• To immobilize glucose oxidase on an electrochemically modified graphite electrode.
• To develop a stable and sensitive amperometric enzyme electrode for glucose detection.
• To characterize the performance of the developed glucose biosensor.

Main Methods:

• Glucose oxidase was immobilized on electrochemically modified graphite.
• Gelatin coating was used to prevent enzyme desorption.
• Amperometric detection of H2O2 generated by the enzyme reaction was employed.
• Electrode performance was evaluated for linearity, selectivity, response time, and stability.

Main Results:

• The enzyme electrode exhibited a linear response to glucose up to 300 microM at 0 mV vs. Ag/AgCl.
• The electrode demonstrated selectivity, with no response to L-ascorbic and uric acid at the working potential.
• A response time of approximately 2 minutes was achieved.
• The enzyme electrode retained about 50% of its initial activity after one week of storage at 4 degrees C.

Conclusions:

• Electrochemical immobilization of glucose oxidase on graphite provides a stable and effective platform for glucose biosensors.
• The developed enzyme electrode offers good linearity, selectivity, and stability for amperometric glucose sensing.
• This approach holds promise for developing practical glucose monitoring devices.