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Experimental supply-demand analysis of anaerobic yeast energy metabolism
O Kroukamp1, J M Rohwer, J H S Hofmeyr
1Department of Biochemistry, University of Stellenbosch, South Africa. 9101705@sun.ac.za
Molecular Biology Reports
|September 21, 2002
Abstract:
Experimental supply-demand analysis of yeast fermentative energy metabolism shows that control of the glycolytic flux is shared between supply and demand. In glucose limited chemostat cultures the supply block was modulated in a dilution rate change and demand block via a benzoic acid titration. Under these conditions the supply block had a flux control of 0.90 and the demand block a flux control of 0.10.