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Updated: Sep 29, 2026
A Mouse Model to Investigate the Role of Cancer-Associated Fibroblasts in Tumor Growth
Published on: December 22, 2020
Skin fibroblasts in vitro radiosensitivity can predict for late complications following AVM radiosurgery
Gijsbert Peter Raaphorst1, Shawn Malone, Ghazi Alsbeih
1Ottawa Regional Cancer Centre, 503 Smyth Road, Ottawa, Ontario K1H 1C4, Canada.
Background And Purpose:
A small proportion of patients undergoing radiotherapy display heightened normal tissue reactions. We have set out to determine whether this sensitivity is genetic in nature and can be assessed using an in vitro skin fibroblast assay in order to predict and avoid excessive normal tissue complications.
Patients And Methods:
In this study we compared five arteriovenous malformation (AVM) patients who were treated with radiotherapy and showed severe normal tissue reactions (necrosis) to two AVM patients who showed normal reactions. Fibroblasts taken from patients were cultured in vitro and irradiated.
Results:
The results showed that the fibroblasts from the sensitive patients were also more radiosensitive in vitro than the cells from the normally responding patients.
Conclusions:
The results suggest underlying genetic radiosensitivity and that such an assay may be used for prediction of severe radiosensitivity in AVM patients.
