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Related Experiment Videos

Multiple DNA-Protein Complexes at a Circadian-Regulated Promoter Element.

I. A. Carre1, S. A. Kay

  • 1National Science Foundation Center for Biological Timing, University of Virginia, Charlottesville, Virginia, 22903.

The Plant Cell
|December 1, 1995
PubMed
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Researchers identified a 36-base pair DNA sequence in Arabidopsis that controls circadian regulation of the CAB2 gene. This region, containing a CCAAT box, binds three transcription factors crucial for clock-controlled gene expression.

Area of Science:

  • Plant molecular biology
  • Circadian rhythms
  • Chlorophyll a/b binding proteins (CAB)

Background:

  • CAB genes encode light-harvesting proteins in chloroplasts.
  • CAB2 gene transcription in Arabidopsis is regulated by a circadian oscillator with near-24-hour oscillations.
  • Previous studies indicated a promoter region (-111 to -38) confers circadian regulation.

Purpose of the Study:

  • To dissect the CAB2 promoter region responsible for circadian regulation.
  • To identify DNA-binding factors involved in clock-controlled transcription.
  • To elucidate the molecular mechanism of circadian control over CAB gene expression.

Main Methods:

  • Deletion analysis of the CAB2 promoter in transgenic tobacco.
  • Characterization of DNA-binding complexes using Arabidopsis whole-cell extracts.

Related Experiment Videos

  • In vivo analysis of cab2::luc fusion constructs in transgenic Arabidopsis.

    • Main Results:

    • A 36-bp sequence within the CAB2 promoter confers circadian regulation.
    • This critical element overlaps a conserved CCAAT box.
    • Five DNA-binding complexes were identified, with three putative transcription factors binding to the 36-bp region.

    Conclusions:

    • A specific 36-bp cis-regulatory element controls CAB2 circadian transcription.
    • This element acts as a docking site for multiple transcription factors.
    • These factors are likely key components in the signal transduction pathway linking the circadian clock to CAB gene expression.