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The transcriptional corepressor MTG16a contains a novel nucleolar targeting sequence deranged in t (16; 21)-positive

André T Hoogeveen1, Stefano Rossetti, Violeta Stoyanova

  • 1Department of Clinical Genetics, Erasmus University, Rotterdam, The Netherlands. hoogeveen@ikg.fgg.eur.nl

Oncogene
|September 21, 2002
PubMed

Insights

Myeloid Translocation Gene (MTG) proteins are novel corepressors. MTG16a

Area of Science:

  • Molecular Biology
  • Cancer Genetics
  • Epigenetics

Background:

  • Myeloid Translocation Gene (MTG) proteins function as transcriptional corepressors.
  • The MTG16 gene is altered in myeloid malignancies by the t(16;21) translocation.
  • MTG16a is a protein isoform encoded by the MTG16 gene.

Purpose of the Study:

  • To investigate the function and localization of MTG16a.
  • To elucidate the role of MTG16a in the pathogenesis of myeloid malignancies.
  • To characterize the AML1-MTG16 fusion protein resulting from the t(16;21) translocation.

Main Methods:

  • Amino acid sequence mapping to determine nucleolar localization.
  • Interaction studies with histone deacetylases (HDACs).
  • Oligomerization assays for MTG proteins and fusion proteins.

Main Results:

  • MTG16a is localized to the nucleolus via its N-terminal region.
  • MTG16a interacts with HDACs, suggesting a role in gene silencing.
  • The AML1-MTG16 fusion protein localizes to the nucleoplasm and interacts with HDAC1 and HDAC3.

Conclusions:

  • Alterations in MTG16a localization and function due to the t(16;21) translocation may contribute to myeloid malignancies.
  • The interaction of the AML1-MTG16 fusion protein with HDACs and its altered localization are critical in disease pathogenesis.
  • Deficiency of nucleolar HDACs and accumulation at AML1-binding sites are implicated in the development of t(16;21) myeloid malignancies.

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