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Published on: June 29, 2014
[Studies on the stability of sodium taurocholate in different media and in the course of sterilization]
Objective:
To investigate the stability of the main active constituent of snake gall sodium taurocholate, TCANa in 25% alcoholic solution and artificial gastric juice, and in the course of hot pressurized sterilization.
Method:
The stability of the solution was predicted by an accelerated isothermal test, and the content of TCANa was determined by TLC-colorimetry.
Result:
The half-time(t1/2) of TCANa in 25% alcoholic solution is 60.57 d at 25 degrees C, and in artificial gastric juice 11.37 d at 37 degrees C, the loss rate under the condition(115 degrees C, 30 min) of sterilization about 3%.
Conclusion:
TCANa in 25% alcoholic solution is unstable. Therefore it is suggested that in order to raise the stability of TCANa the alcoholic concentration be increased and the solution be stored at temperatures below 0 degree C.
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