Related Experiment Video
Updated: Aug 2, 2026
Analysis of Raw and Processed Cyperi Rhizoma Samples Using Liquid Chromatography-Tandem Mass Spectrometry in Rats with Primary Dysmenorrhea
Published on: December 23, 2022
[TLC analysis of harpagoside in devil claw extract and its tablets]
Objective:
To set up a method for TLC identification of the Devil Claw Extract and its tablets, and make a quantitative analysis of harpagoside the main active constituent in the Extract.
Method:
A characteristic TLC chromatogram was obtained by using pre-coated silica gel 60F254 plate, chloroform-ethyl acetate-methanol-water as the solvent system and 5% vanillin-sulfuric acid solution or 10% sulfuric acid-ethanol solution as the spray reagent; and quantitative analysis was carried out using silica gel plate modified by 1% sodium hydroxide, and UV absorptive scanning at 283 nm.
Result:
The regression equation is Y = 20.33X + 92.72, r = 0.9993, linear range between 16.8-151.2 ng, and average recovery 101.2%.
Conclusion:
This method has been validated by linearity, stability, sensitivity and reproducibility tests, and proved to be accordant with the requirement for quantitative analysis of the herbal medicine in question.
