Related Experiment Video

Updated: Aug 9, 2026

Pharmacologic Induction of Epidermal Melanin and Protection Against Sunburn in a Humanized Mouse Model
12:37

Pharmacologic Induction of Epidermal Melanin and Protection Against Sunburn in a Humanized Mouse Model

Published on: September 7, 2013

18.7K

p33(ING1) enhances UVB-induced apoptosis in melanoma cells

K-John Cheung1, Gang Li

  • 1Division of Dermatology, Department of Medicine, University of British Columbia, Vancouver Hospital and Health Sciences Centre, Vancouver, British Columbia, V6H 3Z6, Canada.

Experimental Cell Research
|September 24, 2002
PubMed

Insights

The tumor suppressor ING1 protein (p33ING1) promotes UV-induced apoptosis in melanoma cells. This process requires p53 and involves the mitochondrial pathway, highlighting a potential therapeutic target.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Cell Biology

Background:

  • The ING1 tumor suppressor shares functions with p53, including mediating apoptosis.
  • ING1 isoforms, like p33ING1, translocate to the nucleus and interact with PCNA after UV exposure.

Purpose of the Study:

  • To investigate the role of p33ING1 in UV-induced apoptosis in melanoma cells.
  • To elucidate the mechanism by which p33ING1 influences UVB-induced cell death.

Main Methods:

  • Overexpression and antisense plasmid introduction of p33ING1 in melanoma cells.
  • Assessment of apoptosis rates following UVB irradiation.
  • Analysis of p53 dependence, Bax expression, and mitochondrial membrane potential.

Main Results:

  • Overexpression of p33ING1 increased UVB-induced apoptosis, while antisense inhibition reduced it.
  • p33ING1-mediated apoptosis enhancement was dependent on the presence of p53.
  • p33ING1 upregulated Bax expression and altered mitochondrial membrane potential.

Conclusions:

  • p33ING1 acts as a mediator of UVB-induced apoptosis in melanoma.
  • p33ING1 cooperates with p53 through the mitochondrial cell death pathway in response to UVB.
  • These findings suggest p33ING1 as a potential target for melanoma treatment.

More Related Videos

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells06:09

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

Published on: June 7, 2019

9.4K
Studying Chronic Exposure of Mice to Ultraviolet B Radiation03:20

Studying Chronic Exposure of Mice to Ultraviolet B Radiation

Published on: August 19, 2025

1.8K

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
Mutations01:35

Mutations

Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
Pigmentation01:19

Pigmentation

The color of the skin is influenced by a number of pigments, including melanin, carotene, and hemoglobin. Recall that melanin is produced by cells called melanocytes, which are found scattered throughout the stratum basale of the epidermis. The melanin is transferred to the keratinocytes via melanosomes.
Melanin occurs in two primary forms: eumelanin that provides black and brown pigment and pheomelanin that provides red color. Dark-skinned individuals produce more melanin than those with pale...
Skin Cancer01:30

Skin Cancer

Skin cancer is a type of cancer that occurs when there is an abnormal growth of skin cells, usually triggered by damage to the DNA within the skin cells. It is primarily caused by exposure to ultraviolet (UV) radiation from the sun or artificial sources like tanning beds. Skin cancer is the most common type of cancer worldwide, and its incidence continues to rise.
Basal Cell Carcinoma (BCC): BCC is the most common type of skin cancer, accounting for about 80% of cases. It typically develops in...