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[Gentamicin stability and a method for its quantititive determination].

V D Kartseva, Iu A Portnoĭ, N A Vakulenko

    Antibiotiki
    |June 1, 1975
    PubMed
    Summary
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    Gentamycin stability in acidic solutions follows first-order kinetics and temperature-dependent inactivation. A new polarimetric method accurately quantifies gentamycin levels during purification, correlating well with microbiological assays.

    Area of Science:

    • Pharmaceutical Chemistry
    • Analytical Chemistry
    • Biochemistry

    Context:

    • Gentamycin is a critical antibiotic requiring stability assessment for effective therapeutic use.
    • Understanding degradation pathways is essential for optimizing drug formulation and storage.
    • Accurate quantification methods are needed throughout the antibiotic purification process.

    Purpose:

    • To investigate the stability profile of gentamycin across a range of pH conditions.
    • To elucidate the kinetics of gentamycin inactivation in acidic environments.
    • To develop and validate a novel polarimetric method for gentamycin quantification during purification.

    Summary:

    • Gentamycin inactivation in acidic solutions adheres to first-order reaction kinetics.

    Related Experiment Videos

  • The rate constant for inactivation is temperature-dependent, following the Arrhenius equation.
  • A polarimetric technique was established for assaying gentamycin concentrations from ion-exchange eluates, demonstrating good correlation with microbiological analysis.

    • Impact:

    • Provides crucial data on gentamycin degradation, informing formulation and storage guidelines.
    • Offers a validated, non-destructive analytical method for real-time monitoring of gentamycin purification.
    • Enhances the efficiency and reliability of antibiotic production processes.