Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gluconeogenesis in vertebrate livers.

J W Phillips1, F J Hird

  • 1Russel Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.

Comparative Biochemistry and Physiology. B, Comparative Biochemistry
|January 1, 1977
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hyperammonaemia due to ornithine transcarbamylase deficiency.

Archives of disease in childhood·2010
Same author

Fluctuations in cosmic radiation at radio-frequencies.

Nature·2010
Same author

Studies on fermentation in rice and barley.

American journal of botany·2010
Same author

Genetic inheritance of gene expression in human cell lines.

American journal of human genetics·2004
Same author

Futile cycles revisited: a markov chain model of simultaneous glycolysis and gluconeogenesis.

Journal of theoretical biology·2002
Same author

A benefit of one's own: older women's entitlement to Social Security retirement.

Social security bulletin·2001
Same journal

RETINOID METABOLISM AND CONVERSION OF RETINOL TO DEHYDRORETINOL IN THE CRAYFISH (<i>PROCAMBARUS CLARKII</i>) RETINA.

Comparative biochemistry and physiology. B, Comparative biochemistry·2015
Same journal

Distribution of taurine and other free amino acids in the visual pathway of the crayfish procambarus clarkii.

Comparative biochemistry and physiology. B, Comparative biochemistry·2013
Same journal

Comparative study of free and membrane-bound acidic beta-D-glucosidase from the hepatopancreas of the shrimp Penaeus japonicus (Crustacea: decapoda).

Comparative biochemistry and physiology. B, Comparative biochemistry·2010
Same journal

Purification and characterization of an alpha-glucosidase from the hepatopancreas of the shrimp Penaeus japonicus (Crustacea: decapoda).

Comparative biochemistry and physiology. B, Comparative biochemistry·2010
Same journal

A neutral beta-galactosidase from the hepatopancreas of the shrimp Penaeus monodon (Crustacea: Decapoda): dimeric and sialyated.

Comparative biochemistry and physiology. B, Comparative biochemistry·2010
Same journal

Purification and properties of a beta-mannosidase from shrimp (Penaeus japonicus) hepatopancreas.

Comparative biochemistry and physiology. B, Comparative biochemistry·2010
See all related articles

The liver functions as a gluconeogenic organ, converting lactate from skeletal muscle activity into glucose. This metabolic pathway is crucial for energy recovery in many animal species.

Area of Science:

  • Biochemistry
  • Physiology
  • Comparative Metabolism

Background:

  • Skeletal muscle contraction produces lactate, a metabolic byproduct.
  • The liver's role in lactate metabolism and gluconeogenesis is a key area of physiological research.

Purpose of the Study:

  • To investigate the hypothesis that the liver evolved as a gluconeogenic organ to process lactate from skeletal muscle.
  • To examine the presence and activity of key gluconeogenic enzymes in different animal species.

Main Methods:

  • Measuring lactate levels in skeletal muscle and blood after electrical stimulation.
  • Assessing gluconeogenesis from lactate in isolated liver slices.
  • Detecting the presence of key enzymes: phosphoenolpyruvate carboxykinase and pyruvate carboxylase.

Related Experiment Videos

Main Results:

  • Lactate was detected in skeletal muscle and blood, increasing with electrical stimulation.
  • Liver slices from most animals demonstrated gluconeogenesis from lactate, with exceptions in amphibia.
  • Phosphoenolpyruvate carboxykinase was universally present, while pyruvate carboxylase was absent in toads.

Conclusions:

  • The liver's gluconeogenic capacity from lactate supports the hypothesis of its role in energy recovery from muscle activity.
  • Enzyme distribution suggests variations in metabolic pathways across species, with amphibia potentially utilizing other gluconeogenic routes.