[Immunological characteristics of LPV oncornavirus produced by T-9 human cell line]

The results of immunological study of oncornavirus (LPV strain) produced by a continuous human T-9 cell line are presented. The method of immunodiffusion in agar revealed the following constituent antigens in the virus: (a) those identical to internal and external antigens of Mason-Pfizer oncornaviruses particles; (b) those specific only for LPV virus and not common in other known oncornaviruses of mammals. These antigens were common in LPV virus from T-9 culture and from xenogeneic T-BHK culture in which Mason-Pfizer virus antigens were absent completely; and (c) interspecific antigen of oncornavirus C type present in minimal amounts. Localization of the above antigens in virus particles of LPV oncornavirus was confirmed by the electron immunoenzyme method. Upon separation of virus polypeptides in polyacrylamide gel it was found that the antigens cross-reacting with Mason-Pfizer virus as well as LPV specific antigens were localized both in the main inner virus protein and in the surface glycoprotein.