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Updated: Aug 14, 2026

Electrophoretic Separation of Proteins
08:17

Electrophoretic Separation of Proteins

Published on: June 12, 2008

[Experience with the electrophoretic separation of lipoproteins on Celogel]

P Ilinov, S Torbova, S Popnikolov

    Vutreshni Bolesti
    |January 1, 1975
    PubMed

    Insights

    This study used hydroxycellulose gel electrophoresis to analyze serum lipoproteins in patients with cardiovascular conditions. Findings correlate lipid ratios with lipoprotein profiles, aiding in hyperlipoproteinemia phenotype determination.

    Area of Science:

    • Clinical Chemistry
    • Biochemistry
    • Cardiovascular Medicine

    Context:

    • Serum lipoprotein analysis is crucial for diagnosing and managing dyslipidemias.
    • Routine laboratory methods are essential for practical clinical applications.
    • Cardiovascular diseases, diabetes, and hyperlipoproteinemia often involve altered lipid profiles.

    Purpose:

    • To evaluate the utility of hydroxycellulose gel foils (Cellogel) for routine serum lipoprotein separation.
    • To investigate the relationship between serum lipid levels (cholesterol, triglycerides) and lipoprotein profiles.
    • To explore the potential for determining hyperlipoproteinemia phenotypes using these methods.

    Summary:

    • Hydroxycellulose gel electrophoresis was employed to separate serum lipoproteins in 94 patients with hypertension, coronary disease, essential hyperlipoproteinemia, diabetes, and acute myocardial infarction.
    • Serum total cholesterol and triglycerides were measured and correlated with the ratios of beta-lipoproteins to chylomicrons and pre-beta-lipoproteins.
    • Graphical representations illustrated these correlations, and attempts were made to determine hyperlipoproteinemia phenotypes in affected patients.

    Impact:

    • Provides a practical method for lipoprotein electrophoresis in routine clinical settings.
    • Offers insights into the complex relationships between different lipid fractions and cardiovascular risk.
    • Facilitates a better understanding and classification of hyperlipoproteinemia for improved patient management.

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