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Updated: Sep 29, 2026
Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay
Published on: June 7, 2020
Review of the methods used in the determination of phytoestrogens
Chao-Cheng Wang1, Jeevan K Prasain, Stephen Barnes
1Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294, USA. sam.wang@ccc.uab.edu
Abstract:
Interest in analytical methods for plant estrogens (phytoestrogens) has risen sharply in the past 10 years. In this review, we examine the existing analytical methods based on separations by gas-liquid chromatography, high-performance liquid chromatography and capillary electrophoresis in addition to methods of detection by ultraviolet absorption, fluorescence, electrochemical oxidation/reduction and mass spectrometry. These methods are compared with other methods of phytoestrogen analysis utilizing immunoassay approaches. The advantages and disadvantages of each of these methods are highlighted and potential areas for further development identified.
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