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Different mechanisms are utilized by HIV-1 Nef and staphylococcal enterotoxin A to control and regulate
Nutthapong Tangsinmankong1, Noorbibi K Day, Robert A Good
1Department of Pediatrics, University of South Florida College of Medicine, All Children's Hospital, 801 Sixth Street South, St. Petersburg, FL 33701, USA.
Abstract:
Interleukin-10 (IL-10) plays an important immunopathogenic role in immunologic diseases, especially in HIV infection and atopic dermatitis. The control and regulatory mechanisms of IL-10 production have not been described in these diseases. Recently, we demonstrated that HIV-1 Nef induces IL-10 production in monocytes and that staphylococcal enterotoxin A (SEA) induces IL-10 production in T-lymphocytes. Here we show that Nef-induced IL-10 production and mRNA expression are strongly blocked by rapamycin, but are not blocked by cyclosporin (CsA) or FK506. Conversely, we show that CsA and FK506 completely inhibit SEA-induced IL-10 protein production and mRNA expression. The results of this study demonstrate that IL-10 production by Nef and SEA is controlled and regulated by different mechanisms.