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Endoplasmic Reticulum Forms a Dynamic Continuum for Lipid Diffusion between Contiguous Soybean Root Cells.

S. Grabski1, A. W. De Feijter, M. Schindler

  • 1Department of Biochemistry, Michigan State University, East Lansing, Michigan 48824.

The Plant Cell
|January 1, 1993
PubMed
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Plant cells communicate lipids via the endoplasmic reticulum (ER), not the plasma membrane. Plasmodesmata act as barriers, but ER pathways enable lipid transfer between cells for signaling.

Area of Science:

  • Plant Cell Biology
  • Membrane Biology
  • Lipid Signaling

Background:

  • Intercellular communication in plants relies on plasmodesmata for molecule transport.
  • The roles of the plasma membrane and endoplasmic reticulum (ER) in lipid transport across plasmodesmata are not fully understood.

Purpose of the Study:

  • To investigate the intercellular transport capabilities of lipids and phospholipids.
  • To determine the involvement of the ER and plasma membrane in plant cell communication.

Main Methods:

  • Utilized fluorescence redistribution after photobleaching (FRAP) to study lipid analog mobility.
  • Employed confocal fluorescence microscopy to visualize probe localization in ER and plasma membrane.
  • Synthesized and utilized fluorescent lipid and phospholipid analogs, including NBD-DAG.

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Main Results:

  • Fluorescent probes localized to either the ER or plasma membrane.
  • Probes predominantly in the ER showed intercellular transfer, indicating communication.
  • Probes exclusively in the plasma membrane did not exhibit detectable intercellular communication.
  • A synthesized phospholipid analog degraded to NBD-DAG, which localized to the ER and was transferred between cells.

Conclusions:

  • The ER, not the plasma membrane, forms dynamic lipid communication pathways between plant cells.
  • Plasmodesmata act as barriers to lipid diffusion within the plasma membrane.
  • Lipid signaling molecules, like diacylglycerol, can be transferred between plant cells via the ER.