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Related Experiment Videos

Arabidopsis GLABROUS1 Gene Requires Downstream Sequences for Function.

J. C. Larkin1, D. G. Oppenheimer, S. Pollock

  • 1School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska 68588-0118.

The Plant Cell
|December 1, 1993
PubMed
Summary

The GLABROUS1 (GL1) gene in Arabidopsis is crucial for initiating trichome development. A downstream enhancer in the 3' noncoding region is essential for its normal expression pattern.

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Trichome Development in Arabidopsis thaliana. I. T-DNA Tagging of the GLABROUS1 Gene.

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Area of Science:

  • Plant Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • The Arabidopsis GLABROUS1 (GL1) gene, a myb gene homolog, is essential for trichome initiation.
  • Previous studies indicated GL1 expression in stipules based on 5' noncoding region promoter activity.

Purpose of the Study:

  • To investigate the regulatory elements controlling GL1 gene expression.
  • To identify sequences responsible for the specific expression pattern of GL1.

Main Methods:

  • In situ hybridization to determine GL1 transcript localization.
  • Reporter gene assays (GUS) with deletion analysis of GL1 noncoding regions.

Main Results:

  • In situ hybridization showed high GL1 transcript levels in developing trichomes.

Related Experiment Videos

  • Deletion analysis identified a crucial enhancer in the 3' noncoding region of GL1.
  • Combined upstream and downstream sequences directed GUS expression in leaf primordia, developing trichomes, and stipules.

    • Conclusions:

    • A downstream enhancer in the 3' noncoding region is essential for the normal expression pattern of the GL1 gene.
    • This enhancer, along with upstream sequences, ensures GL1 is expressed in leaf primordia, developing trichomes, and stipules.