Steroid receptors in Streptomyces hydrogenans: isolation and characterization of a high affinity receptor for 5alpha-dihydrotestosterone
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Updated: Oct 21, 2026

Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding
Published on: September 21, 2011
Abstract:
There are different steroid binding receptors in the cytosol of Streptomyces hydrogenans. A high molecular weight component binds 5alpha-dihydrotestosterone (17beta-hydroxy-5alphaH-androstan-3-one) with high affinity. Partial purification is achieved by density gradient centrifugation and filtration on Sephadex G-200. 5alpha-Dihydrotestosterone and progesterone compete for the same binding sites on the receptor. Cyproterone and testosterone are not bound. As it is easy to isolate the binding fraction, a simple assay for 5alpha-dihydrotestosterone or progesterone in biological samples is available.
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