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Related Experiment Videos

Evaluation of methods for total selenium determination in yeast.

Nadine E Renard1, Thomas A Tompkins

  • 1Institut Rosell, Montréal, Québec, Canada.

Biological Trace Element Research
|September 26, 2002
PubMed
Summary

Determining selenium in biological samples is challenging. Inductively coupled plasma-mass spectrometry (ICP-MS) and flame atomic absorption spectrometry (FAAS) with specific digestion methods offer reliable results for Se yeast analysis.

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Area of Science:

  • Analytical Chemistry
  • Environmental Science
  • Biochemistry

Background:

  • Classical fluorometric methods (FM) for selenium determination in biological samples are hazardous and time-consuming due to aggressive sample digestion.
  • Commercial labs increasingly use faster instrumental methods, often avoiding perchloric acid (HClO4) in digestion.
  • Changes in digestion protocols may compromise selenium analysis reliability, particularly for organic selenium forms like those in Se yeast.

Purpose of the Study:

  • To evaluate the applicability of different instrumental methods and sample digestion techniques for selenium analysis in Se yeast.
  • To compare results from flame atomic absorption spectrometry (FAAS), inductively coupled plasma-mass spectrometry (ICP-MS), and hydride generation atomic absorption spectrometry (HGAAS) against classical FM and instrumental neutron activation analysis (INAA).

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Main Methods:

  • Samples of Se yeast and selenomethionine standards were analyzed using FAAS, ICP-MS, and HGAAS.
  • Sample digestion methods included microwave digestion with HNO3/H2O2, and digestion with HNO3/HCl.
  • Results were benchmarked against fluorometric method (FM) and instrumental neutron activation analysis (INAA).

Main Results:

  • ICP-MS with microwave digestion (HNO3/H2O2) yielded results within 5% of expected values, comparable to FM and INAA.
  • FAAS achieved acceptable results after digestion with HNO3/HCl.
  • HGAAS with HNO3/H2O2 digestion showed systematically elevated values (~10% higher) with high standard deviations (>10%).

Conclusions:

  • Microwave digestion using HNO3/H2O2 is suitable for ICP-MS analysis of selenium in Se yeast.
  • FAAS with HNO3/HCl digestion is also applicable for reliable Se yeast analysis.
  • HGAAS is not recommended for Se yeast analysis with current HNO3/H2O2 digestion protocols due to inaccurate and imprecise results.