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Updated: Aug 13, 2026
A Simple and Efficient Method to Detect Nuclear Factor Activation in Human Neutrophils by Flow Cytometry
Published on: April 9, 2013
Demonstration of an activation regulated NF-kappaB/I-kappaBalpha complex in human platelets
Fengqi Liu1, Steve Morris, Jerry Epps
1Department of Anesthesiology, Graduate School of Medicine, University of Tennessee Medical Center, 1924 Alcoa Highway, Knoxville, TN 37920, USA.
Abstract:
In eukaryotic cells, the ubiquity of the signaling system of transcription factor nuclear factor-kappa B (NF-kappaB)/I-kappa B (I-kappaB) is undisputed. Numerous studies have reported that the NF-kappaB/I-kappaB complex plays a pivotal role in regulating gene expression controlling cell differentiation, cell proliferation, inflammation, oncogenesis, and apoptosis. Here we show that NF-kappaB/I-kappaB families exist in human platelets, natural anuclear corpuscles derived from megakaryocytes. Moreover, the I-kappaB kinase (IKK) is present and may phosphorylate I-kappaB during platelet activation. Coupled with intracellular calcium flux, this leads to I-kappaB dissociation from the NF-kappaB/I-kappaB complex and proteolysis. The NF-kappaB/I-kappaB proteins may have function independent of gene regulation in platelets.
