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Updated: Aug 17, 2026

Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology
Published on: March 23, 2011
CTLA-4 upregulation during aging
Qibin Leng1, Zvi Bentwich, Gadi Borkow
1Ruth Ben-Ari Institute of Clinical Immunology and AIDS Center, Kaplan Medical Center, Hebrew University Hadassah Medical School, 76100, Rehovot, Israel.
Abstract:
The immune system gradually becomes anergic with age. Here, we measured intracellular levels of cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4), a negative regulator of T-cells, in 53 healthy individuals aged 18-94. We found a highly significant correlation between age and percent of CTLA-4+CD4+ cells (r=0.6, P<0.001) and between age and mean fluorescence intensities of CTLA-4 (i.e. number of molecules, r=0.61, P<0.001). CTLA-4 levels were also correlated with immune activation, determined by levels of HLA-DR+CD3+ cells (r=0.55, P<0.001). We postulate that immune senescence associated with age is caused in part by chronic immune activation with related decrease in CD28 costimulatory molecules and increase in inhibitory CTLA-4 molecules.
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