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[Identification of differentially expressed genes in bone marrow CD34+ cells by suppression subtractive
Jie Chen1, Shu-yun Zhou, Ru Feng
1Department of Hematology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. chinsyou@263.net
Objective:
To understand the differential gene expression profiles between bone marrow cells and mobilized peripheral blood CD34(+) cells.
Methods:
Suppression subtractive hybridization (SSH) was employed to identify the genes differentially expressed in bone marrow and mobilized peripheral blood CD34(+) cells obtained from a healthy donor.
Results:
Twenty-one differentially expressed genes were identified that could be categorized into S-phase- or G(2)-M cell cycle-related genes and CCAAT/enhancer binding protein (C/EBP) transcription factor family. The genes highly expressed in bone marrow CD34(+) cells indicated low expression of their counterparts as SSH demands strict paired comparison between the 2 types of CD34(+) cells of different origins.
Conclusion:
CD34(+) cells derived from bone marrow are featured by more active proliferation than are those from mobilized peripheral blood.