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Related Experiment Videos

Calcium Requirement for Ethylene-Dependent Responses.

V. Raz1, R. Fluhr

  • 1Department of Plant Genetics, Weizmann Institute of Science, P.O.B. 26, Rehovot, Israel 76100.

The Plant Cell
|September 1, 1992
PubMed
Summary

Calcium ions are essential for plant hormone ethylene

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Ethylene Signal Is Transduced via Protein Phosphorylation Events in Plants.

The Plant cell·1993

Area of Science:

  • Plant Biology
  • Plant Hormone Signaling
  • Biochemistry

Background:

  • Ethylene is a crucial plant hormone regulating development, defense, and fruit ripening.
  • Ethylene perception involves a specific receptor, but downstream signaling pathways remain unclear.
  • Plant pathogenesis response, like chitinase gene induction, serves as a model for studying ethylene signaling.

Purpose of the Study:

  • To investigate the role of calcium in ethylene-dependent signal transduction pathways.
  • To elucidate the necessity of calcium fluxes in ethylene-mediated plant responses.
  • To understand calcium's involvement in ethylene's control over pathogenesis and growth.

Main Methods:

  • Utilized the induction of pathogenesis-related gene chitinase as a model system.
  • Manipulated calcium levels using chelators, ionophores (ionomycin), and pump blockers (thapsigargin).
  • Assessed ethylene response in plants grown in calcium-deficient soil and restored sensitivity with calcium addition.

Main Results:

  • Calcium fluxes are necessary for ethylene-dependent chitinase induction.
  • Increased cytosolic calcium stimulated chitinase accumulation.
  • Calcium-deficient plants showed impaired ethylene responses, including pathogenesis and abscisic acid-controlled guard cell closure.
  • Restoring calcium levels in deficient plants re-established ethylene sensitivity.

Conclusions:

  • Calcium is a critical component required for diverse ethylene-dependent processes in plants.
  • Calcium acts as a necessary second messenger in ethylene signal transduction.
  • Understanding calcium's role provides insights into plant hormone crosstalk and stress responses.

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